*Ctr for Magn Reson Res, Depts of Radiology and Biomedical Eng, Univ of Minnesota, USA +Inst of Math Modeling, Danish Tech Univ, Denmark
This study was an attempt to disgiinguish the motor regions of the human brain involved in planning motor movement from those involved in its execution, using single-trial fMRI. Since the behavioural response during such a motor experiment is inconstant from trial to trail, K-means clustering (1) following finite impulse response (FIR) filtering (2), was employed to extract the above information from fMRI data.
Materials and Methods
The paradigm consisted of a delayed, cured joystick movement task. The subject was required to move a cursor from the centre of the screen to the memorized location of a target, after a delay period following target presentation. Each run consisted to 16 such repetitions, or trails, with variable delays (0-3 sec) and target-locations. The intertrial interval was 20 sec. Behavioural data including reaction and movement times, were recorded. The study was conducted on normal adult human subjects using a 4 Telsa MRI system. 10 coronal sections of thickness 5 mm each were imaged, including the primary, supplementary and pre-motor areas. 64X64 size single-shot EPI image wer acquired every 100ms (1 volume/sec).
Analysis and Results
Data analysis was done using the Lyngby toolbox(http://hendrix.imm.dtu.dk/software.software.html). The means of each trial in a run was zeroed. Two paradigm functions were constructed for analysis based on the behavioural data: one for motor preparation, the other for execution. FIR filtering was done on the time-courses using these two reference functions, and K-means clustering was performed on these filters. The non-noise clusters had FIR filters that in the majority were of the form of a single smooth, positive wave, differing primarily in the latency and amplitude of the response.
The voxels in the motor area clearly showed differences in activation in terms of temporal relation to the planning and execution phase of a motor task. FIR filtering followed by K-means clustering on the coefficients, provide a good tool not only for separate voxels with different temporal activation patterns in single-trial experiments but also to visualize the BOLD response.
Supported by NIH (MH57180 and RR08079).
1. Goutte, C. et al. Neuroimage, 1998, 7: S610;
2. Nielsen, F.A. et al. Neuroimage, 1997, 5: S473